Description
The Lambert Instruments LIFA system is the fastest and easiest way to perform Fluorescence Lifetime Imaging Microscopy (FLIM).
Available in versatile configurations dependent on your specific applications, the LIFA system is a turn-key solution for fluorescence lifetime imaging microscopy. Compatible with any fluorescence microscope with a camera output – including microscopes by Leica, Nikon, Olympus, TILL and Zeiss. Set up is quick and easy, with all hardware integrated seamlessly with dedicated LIFA software, so you can focus on your experiment.
The advanced software instantly analyses data and presents the calculated fluorescence lifetimes visually. Recorded images are compatible with ImageJ, FIJI, Matlab and MetaMorph, while detailed statistical data can be exported to an Excel worksheet.
Features
Experiments from start to finish
From recording images to lifetime calculations and data analysis
Dedicated LIFA software
Instantly calculates the fluorescence lifetime and presents it as a color-coded overlay
Broad Lifetime Range
From sub microsecond down to picoseconds
Multiple configurations
System components to suit your applications
Non-phototoxic illumination
LIFA vTAU
SPAD powered FLIM system
Simplify experiments for researchers and imaging centers with the vTAU SPAD camera; combining excellent light sensitivity with easy image acquisition and data analysis.
Minimize measurement duration, automate image acquisition, and simplify data analysis… factors of great importance for cell biology, cancer research and high-throughput screening.
vTAU easily integrates into any FLIM system, providing a plug-n-play experience that allows for seamless switching between setups.
Key features
Unique image sensorUltra-high sensitive SPAD detector for up to 100 lifetime images per second
Multiple image modesIncluding regular Frequency-Domain FLIM acquisition and Time-Lapse recordings
Characteristics
Pixel resolution: 512 x 512 px
Pixel size: 16 μm
Lifetime range: 0.2 to 300ns
Frame rate: up to 300 fps
Sensor type: SPAD
System Components
The LIFA vTAU system includes:
Dedicated LIFA Software
Light source(s) according to your application
Computer with USB connection
On-site system installation
1 day of hands-on training
Phone, email and remote desktop support
LIFA Software
Seamless integration of hardware for full system controlGuides user through FLIM experiments from start to finish.
Supports third-party hardware for a flexible and expandable system.
Light sources
Multi-LED and/or Multi-LASER as required for your set up
Excitation light sources for frequency-domain fluorescence lifetime imaging, supplied according to your required wavelengths.
User Publications
- Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic β-cateninJuly 19 – FLIM
- Electrocatalytic Reaction Driven Flow: Role of pH in Flow ReversalMar 2, 2022 – FLIM, proton concentration, University of Twente
- Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neuronsDec 7, 2021 – Microscopy, Fluorescence
- Quantification of PD-1/PD-L1 Interaction between Membranes from PBMCs and Melanoma Samples Using Cell Membrane Microarray and Time-Resolved Förster Resonance Energy TransferDec 7, 2021 – FRET, Fluorescence
- Glycerol suppresses glucose consumption in trypanosomes through metabolic contestAug 13, 2021 – FLIM
- PIM kinases inhibit AMPK activation and promote tumorigenicity by phosphorylating LKB1Jun 30, 2021 – FLIM
